Skip to main content
. Author manuscript; available in PMC: 2009 Apr 27.
Published in final edited form as: J Neurochem. 2008 Feb 4;105(5):1820–1833. doi: 10.1111/j.1471-4159.2008.05279.x

FIGURE 5. AMPK activator does not restore/attenuate psychosine mediated activation of cell death in MO3.13.

FIGURE 5

To examine cell viability, MO3.13 cells were plated and treated with AICAR (0.1-2.0mM) followed by psychosine treatment (20 μM) for 24 h. Viable cells were determined by MTT assay (A) and cell supernatant was processed for LDH assay (B) as described in Material and Methods. Data are the mean ± SD of six values. *** P<0.001 as compared with untreated cells; and NS, not significant as compared with psychosine-treated cells. Cells were treated as shown in figure and lysates were prepared and subjected to caspase 3 activity assay determined by measuring the fluorescence intensity (expressed as arbitrary U/mg protein) of liberated amino-4-methyl-coumarin using spectrofluorometry as described as in Materials and Methods (C). Data are mean ± SD of six values. *** P<0.001 compared with untreated cells; and NS, not significant as compared with psychosine-treated cells. MO3.13 cells were treated as in Materials and Methods and at 24 h were lysed in DNA lysis buffer; the extracted DNA obtained was electrophoresed on a 2% agarose gel. H2O2 (200μM) was used to induce DNA fragmentation as a control (D). Gel is representative of two individual experiments.