Figure 3.

mAb 2C12 abrogates import block. (A) MxA-expressing 3T3 cells were microinjected with a mixture of viral nucleocapsids and antibody 2C12 (9). Microinjected cells were kept for 2 h in the presence of CHX and then analyzed for the localization of the antibody (a; green) or the viral NP protein (b; red). (B) MxA-expressing 3T3 cells were microinjected with either antibody 2C12 (a and c) or an unrelated control antibody (b and d), and were infected 8 h later with THOV at a multiplicity of 50 per cell. Cells were fixed 16 h later and analyzed for viral protein synthesis by double-immunofluorescence labeling using a hyperimmune anti-THOV antiserum (c and d). The microinjected antibodies were detected with the help of a fluorescein-conjugated goat anti-mouse IgG antibody (a and b).