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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4865–4873. doi: 10.4049/jimmunol.0801974

FIGURE 4.

FIGURE 4

Confirmation of DENV-derived CD8+ T cell epitope identification in wild-type and IFN-α/βR-/- mice by ICS. A and B, Splenocytes harvested from wild-type (A) or IFN-α/βR-/- (B) mice 7 days after infection with 1011 or 1010 GE of S221, respectively, were restimulated in vitro with individual DENV peptides or an irrelevant peptide. Cells were then stained for surface CD8 and intracellular IFN-γ and analyzed by flow cytometry. The response to the irrelevant peptide was subtracted from the response to each DENV peptide, and the number of CD8+ T cells producing IFN-γ is indicated. Results are expressed as the mean ± SEM of 13 wild-type and 7 IFN-α/βR-/- mice tested in at least three independent experiments. C and D, Kinetics of the DENV-specific CD8+ T cell response. Wild-type mice (C, n = 4) and IFN-α/βR-/- mice (D, n = 3) were infected with 1011 or 1010 GE of S221, respectively, and blood lymphocytes were isolated at various time points. Stimulation and ICS were performed as in A and B and the percentage of CD8+ T cells producing IFN-γ is shown.