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. 2008 Oct 28;364(1514):163–173. doi: 10.1098/rstb.2008.0143

Figure 3.

Figure 3

Impact of 8Br-cGMP and p38 MAPK on SERT activity of autism-associated hSERT-coding variants. (a) Activity modulation. HeLa cells transfected with hSERT or autism-associated hSERT-coding variants were examined for 5-HT transport activities as described in §2 following pretreatments of cells with either 100 μM 8Br-cGMP or vehicle for 1 hour. Parallel wells were treated with the PKG inhibitor H8 (10 μM) to validate specificity. (b) Altered p38 MAPK-dependent regulation of hSERT in transfected HeLa cells. The cells transfected with hSERT or autism-associated hSERT-coding variants were examined for 5-HT transport activities as described in §2 following pretreatments of cells with either 1 μM anisomycin or vehicle for 10 min. Parallel wells were treated with the p38 MAPK inhibitor SB203580 (1 μM) to validate specificity. Results reflect mean±s.e.m. of three separate experiments normalized to each mutant's control measured under vehicle-treated conditions (100%). Results in (a,b) reflect mean ±s.e.m. of three separate experiments normalized to each mutant's level under vehicle-treated conditions (100%). Data were analysed by a one-way ANOVA with post hoc Bonferroni tests comparing variant with hSERT 8Br-cGMP/anisomycin responses, with *p<0.05 taken as significant.