Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Expression of cyclin A, p27Kip1, and p21Cip1 in adherent and nonadherent 32D or 32Dp210 cells. Cells were seeded on fibronectin-coated dishes. Cell extracts from adherent and nonadherent fractions were prepared 20 hr after seeding. Extracts containing 12.5 μg of protein were separated on a 12.5% polyacrylamide gel, and cyclin A, p27Kip1, and p21Cip1 were detected by immunoblotting and enhanced chemiluminescence.
