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Adhesion dependence of cyclin A-associated kinase activity in 32D and 32Dp210 cells. Kinase activity associated with cyclin A immunoprecipitates was detected by using histone H1 as substrate in the presence of 32P-labeled ATP. Labeled histone H1 was separated by PAGE and detected by autoradiography. Cyclin A was immunoprecipitated from cell extracts prepared 20 hr after seeding of 32D or 32Dp210 cells on fibronectin-coated dishes.
