Table 1.
Core Subunits of the Neural BAF SWI/SNF-like ATP-dependent Chromatin Remodeling Complexes as Identified by a Proteomics Approach
| BAF subunits | # peptides differenta (uniqueb) |
Coverage (% mass) | Accessions | p scores |
|---|---|---|---|---|
| BAF250a/b;ARID1a/b/Osa1 | 75 (17) | - | c | 1.00 |
| ARID2 | 13 (8) | 15.4 | d | 1.00 |
| BAF190/Brm/Smarca2 | 10 (4) | 17.5 | NP_035546 | 1.00 |
| BAF190/Brg/Smarca4 | 50 (0) | 23.1 | NP_035547 | 1.00 |
| BAF180/polybromo-1 | 18 (4) | 20.9 | NP_240329e | 1.00 |
| BAF170/Smarcc2 | 34 (2) | 31.3 | NP_937803 | 1.00 |
| BAF155/Srg3/Smarcc1 | 15 (0) | 14.0 | NP_033237 | 1.00 |
| BAF60a/Smarcd1 | 10 (1) | 17.9 | NP_114030 | 1.00 |
| BAF60c/Smarcd3 | 7 (1) | 18.1 | NP_080167 | 1.00 |
| BAF57/Smarce1 | 15 (0) | 49.6 | NP_065643 | 1.00 |
| BAF53a/Actl6a | 11 (9) | 29.3 | NP_062647 | 1.00 |
| BAF53b/Actl6b | 12 (4) | 30.5 | NP_113581 | 1.00 |
| BAF45a/PHF10 | 5 (2) | 15.0 | NP_077212 | 1.00 |
| BAF45b/DPF1 | 22 (2) | 43.0 | NP_038902 | 1.00 |
| BAF45c/DPF3 | 7 (3) | 27.1 | AAH48572 | 1.00 |
| BAF45d/DPF2 | 8 (1) | 30.2 | NP_035392 | 1.00 |
| BAF47/Ini1/mSnf5/Smarcb1 | 8 (0) | 28.6 | NP_035548 | 1.00 |
| BAF42/actinf | 13 (4) | - | - | - |
Number of different peptides for each protein entry.
Number of peptides corresponding to a unique protein entry in the IPI human/mouse databases (Nesvizhskii et al., 2003).
The protein sequence of mouse ARID2 (AT-rich interactive domain 2) will be submitted to NCBI. ARID2 is an evolutionarily conserved protein of 1161 amino acids with a typical ARID DNA-binding domain characteristic of a protein family that includes Drosophila OSA/eyelid, yeast Swi1p, yeast Rsc9 and mammalian BAF250a/ARID1a and BAF250b/ARID1b. Drosophila BAP170, a recently identified subunit of the SWI/SNF-like BAP complex is 43% similar and 26% identical to human ARID2 and most likely represents its orthologue in flies. From an independent purification, human ARID2 was identified and termed BAF200 (Yan et al., 2005).
Rattus norvegicus Pb-1 protein sequence.
α-, β- and γ-actin family of proteins (J.L. and G.R.C, unpublished results).