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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Dev Biol. 2009 Feb 9;328(2):410–421. doi: 10.1016/j.ydbio.2009.01.043

Fig. 2.

Fig. 2

Spoltud-1 immunohistochemistry on adult and juvenile planarians shows localization to chromatoid bodies in neoblasts and testes and perinuclear granules in CNS. (A-D) Confocal maximum projection of an in situ hybridization and immunohistochemistry of histone H2B and spoltud-1. (A) histone H2B mRNA. (B) spoltud-1 protein. (C) nuclei. (D) Overlay image. (E) Deconvolution of a confocal series of a neoblast immunolabeled with anti-spoltud-1. Labeled particles are perinuclear and are all around the nucleus. (F-H) Immunohistochemistry on electron microscope sections. (F) Low magnification of a neoblast cell displaying a chromatoid body. (G) Higher magnification of (F) showing an immunolabeled chromatoid body. (H) Detail of a chromatoid body immunolabeled with anti-spoltud-1. (I-K, M-O) Confocal maximum projection of an anti-spoltud-1 immunohistochemistry on brain sections (I-K) and testis section (M-O). (I, M) spoltud-1 protein. (J, N) Nuclei. (K, O) Overlay images. (L, P) Deconvolution of a confocal section of an anti-spoltud-1 immunohistochemistry on brain sections (L) and testis (P). Arrowheads in L indicate neural processes. nu, nucleus; cb, chromatoid body; mc, mitochondria; sg, spermatogonia; st, spermatids; sp, sperm cells. Scale bars: 5 μm in A-E; 1 μm in F; 400 nm in G; 100 nm in H; 20 μm in I-K; 7.5 μm in L;25 μm in M-O; 7 μm in P.