Spoltud-1 protein is lost after 3 weeks of treatment. (A-L) Anti-Spoltud-1 immunohistochemistry in control and Spoltud-1 (RNAi) animals after second round of injection (3 weeks). (A-C) Parenchyma of a gfp(RNAi) control animal. (D-F) Brain of a gfp(RNAi) control animal. (G-I) Parenchyma of a Spoltud-1 (RNAi) animal. (J-L) Brain of a Spoltud-1 (RNAi) animal. (A, D, G, J) Nuclei staining (blue). (B, E, H, K) anti-Spoltud-1 (green). (C, F, I, L) Overlay images. Anterior is to the bottom left. Scale bars: 30 μm.