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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Dev Biol. 2009 Feb 9;328(2):410–421. doi: 10.1016/j.ydbio.2009.01.043

Fig. 7.

Fig. 7

Neoblasts are rapidly lost after 7 weeks of treatment. (A-L) Confocal maximum projection of a Histone H2B in situ hybridization and anti-PCNA immunohistochemistry in control and Spoltud-1(RNAi) representative animals. (A-D) gfp(RNAi) control after third round of injection (5 weeks). (E-H) Spoltud-1(RNAi) animal after third round of injection (5 weeks). (I-L) Spoltud-1(RNAi) animal after fourth round of injection (7 weeks). (M-R) Confocal maximum projection of an anti-PCNA immunohistochemistry in sagital sections of control and Spoltud-1(RNAi) representative animals. (M-O) gfp(RNAi) animal. (P-R) Spoltud-1(RNAi) animal. (A, E, I, M, P) Nuclei staining (blue). (B, F, J, N, Q) anti-PCNA (green). (C, G, K) Histone H2B fluorescent in situ hybridization (red). (D, H, L, O, R) Overlay images. Anterior is to the left. Scale bars: 50 μminA-H; 30 μmin I-L; 200 μmin M-R.