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. Author manuscript; available in PMC: 2010 May 10.
Published in final edited form as: Virology. 2009 Mar 27;387(2):473–481. doi: 10.1016/j.virol.2009.02.046

Figure 1. Schematic overview of the RHGP Gene Search Vector.

Figure 1

(A) An overview of the pRHGP22 Gene Search Vector (GSV) is shown, highlighting important features of the vector that facilitate the RHGP-based identification of host-based targets. Note that the RSV E/P of the GSV is lost after the integration into the host genome. (B) Shown is an overview of potential outcomes of GSV integration into the genome. The left panels demonstrate how integration of the Gene Search Vector in an “antisense orientation” would disrupt Allele 1 and then facilitate overexpression of an antisense to disrupt the second allele. On the other hand, the right panels demonstrate how integration in a sense orientation would facilitate overexpression of the entire target gene (or domains thereof). Note that the phenotype is tightly regulated by the Tet-inducible promoter, thus allowing the investigation to reverse the phenotype by turning the GSV vector “off” when cells are cultured in the presence of Doxycycline.