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Number of fragments from IR-AI ECD of ubiquitin cations versus laser fluency for (a) and (b) 85 ms IR activation followed by 10 ms ECD of the 6+ and 7+ ions, respectively, and (c) and (d) 10 ms ECD followed by 100 ms IR activation of the 6+ and 7+ ions, respectively. Squares represent the number of c' and z• ions, triangles for c• and z' ions, and circles for b and y ions.
