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Fragmentation diagrams for (a) ECD and (b)–(e) AI ECD of ubiquitin (M + 6H)6+ ion. (b) and (c) IR activation before ECD, ECD delay 85 ms, IR duration 85 and 25 ms, respectively; (d) 100 ms IR activation immediately after ECD; (e) IR activation before ECD, ECD delay 1165 ms, IR duration 85 ms.
