FIG. 3.

RANKL-mediated osteoclastogenesis in wild-type and various knockout pOcs. Wild-type pOcs were compared to pOcs with targeted deletions of either IL-6 (IL-6^−/−), TNF receptors (TNFR1^−/−, TNFR2^−/−, TNFR1/2^−/−), or IL-1 receptor 1 (IL-1R1^−/−) in their ability to form Ocs by exposing them to RANKL at 100 ng/ml for 6 days in the presence of M-CSF (10 ng/ml). There were no differences in the abilities of any of these cell types to form Ocs under these conditions (overall p = 0.105 by one-way ANOVA with post hoc Bonferroni test). All data are expressed as the mean ± SD of at least three experiments performed in triplicate wells.