FIG. 2.

Cepharanthine demonstrates significant protection against neomycin-induced hair cell loss. A Five days post-fertilization zebrafish pre-exposed to cepharanthine for 1 h and then treated with 200 μM neomycin demonstrate significant dose-dependent protection by cepharanthine (**p < 0.01, one-way ANOVA). B Five days post-fertilization zebrafish pre-exposed to 100 μM cepharanthine and then treated with 0, 100, 200, or 400 μM neomycin. Solid line Zebrafish pretreated with cepharanthine prior to neomycin. Dotted line Zebrafish with no pretreatment prior to neomycin. Cepharanthine pretreatment led to significant protection against all doses of neomycin (p < 0.0001, two-way ANOVA). For both graphs, data points represent mean hair cell survival of 10–15 fish. Error bars = ±1 SD from the mean. Data for remaining six protective drugs are presented in Tables 2 and 3.