FIG. 5.

Examples of normal and blocked uptake of Texas-Red conjugated gentamicin (TR-Gent) in the zebrafish lateral line. Hair cells are labeled with YO-PRO1 (green) to label hair cell nuclei, and TR-Gent (red). A Normal uptake of TR-Gent demonstrates multiple double-labeled hair cells. B Uptake of TR-Gent is blocked by pretreatment with the candidate protective drug, phenoxybenzamine. No double-labeled hair cells are seen. Similar findings were seen with carvedilol, hexamethyleneamiloride, and amsacrine. Scale bar in B = 10 μM and applies to both panels.