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. 2009 Mar 3;10(2):205–219. doi: 10.1007/s10162-009-0160-4

FIG. 8.

FIG. 8.

GT immunolabeling and GTTR in the organ of Corti. A Three hours after 2 mg/kg GT injection i.p., GT was immunolocalized in stereociliary bundles (arrowheads) and apices of outer hair cells (OHCs), and in the apices of adjacent pillar and Deiters’ cells. B The cell bodies of OHCs, pillar cells (p), the remaining supporting cells are labeled with approximately equal fluorescent intensity. IHC stereocilia (arrowheads) can also be visualized in this focal plane. C as are nerve fibers (n) proximal to the habenula, transverse (arrowheads), inner spiral bundle fibers (isb), and three rows of outer spiral bundles (osb). D Thirty minutes after GTTR injection i.p., GTTR fluorescence was localized in the hair bundles of both OHCs and IHCs (arrowheads and in the apices of pillar cells (p). E The cell bodies of OHCs, IHCs, pillar cells (p), the remaining supporting cells, including the Deiters’ cell phalangeal processes (arrowheads) are labeled with approximately equal fluorescent intensity. F The interdental cells of the spiral limbus also show GTTR fluorescence at this time point. All tissues from basal coil. Images acquired and post-processed identically.