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. 2009 May 7;4(5):e5473. doi: 10.1371/journal.pone.0005473

Figure 5. RNP reconstitution assays in chicken DF1 cells and human 293T cells. mRNA, cRNA and vRNA levels were measured by primer extension.

Figure 5

(A) RNA levels of RNP derived from WSN (W), HK (H), or from the (PA-H) hybrid of WSN, or from 2 hybrids (PA-W/H or PA-H/W) containing the PA chimeras, were compared in DF1 cells. (B) RNA levels of RNP derived from WSN (W) and HK (H) were compared in human 293T cells. (C) RNA levels of RNP derived from PA-H, PA-W/H and PA-H/W were compared in 293T cells. (D) RNA levels of RNP derived from WSN (W) and Fujian (F) in DF1 cells. (E) RNA levels of RNP derived from WSN (W) and Fujian (F) in 293T cells. In all experiments, activities are expressed as a % relative to the wild-type WSN (W) from 3 independent experiments. Black, white and oblique lined columns show steady-state levels of mRNA, cRNA and vRNA, respectively. * and ** show statistically significant differences from wild-type WSN (W) at p<0.05 and p<0.01 in a Student's t-test. WSN NP was used for WSN and HK strains, and FJ NP was used for the FJ strain.