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. 2009 May 6;4(5):e5449. doi: 10.1371/journal.pone.0005449

Figure 5. Suppressing conditions are associated with decreased SNARE complex formation.

Figure 5

Cells grown under alkaline conditions have reduced levels of SNARE complexes. HA-SEC9 cells were grown in rich media buffered to pH 6.0 or pH 7.5. Lysates from these cells were prepared and levels of SNARE complex formation assayed by immunoprecipitation with antibodies against HA-Sec9p and subsequent western blotting for Snc1/2p and Sso1/2p. Input lanes (I) represent 2% of the lysate that was immunoprecipitated and loaded in the pellet lanes (P). The relative amount of Sso1/2p and Snc1/2p that is co-immunoprecipitated with HA-Sec9p is reduced in lysates from cells grown at pH 7.5 relative to the amount of precipitated SNAREs from cells grown at pH 6.0.