Skip to main content
. 2009 May 6;4(5):e5449. doi: 10.1371/journal.pone.0005449

Figure 6. Decreased growth suppresses the temperature-sensitivity of sec9 alleles.

Figure 6

Serial dilutions of sec9-7, sec9-4, or wild-type cell suspensions were spotted onto rich media containing glucose (YP+glucose), synthetic complete media (SC) or rich media with glycerol (YP+glycerol) and incubated at the indicated temperature for 3 days (YP+glucose and SC) or 5 days (YP+glycerol). On YP+glucose, sec9 mutants are unable to grow at temperatures above 35°C and show decreased growth at 30°C, relative to wild-type cells. Compared to YP+glucose, the growth of wild-type cells is decreased when plated on SC or YP+glycerol and under these conditions both sec9-4 and sec9-7 cells show increased growth at semi- and non-permissive temperatures. The relative increase in fitness associated with growth on YP+glycerol is higher for sec9-7 mutants compared to sec9-4 mutant cells.