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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Biochemistry. 2009 Apr 28;48(16):3658–3668. doi: 10.1021/bi802339c

Table 4.

Comparison of the Thermodynamic Parameters of F159W-Leuko- and native PNPs at 25 °C

 
 F159W-Leuko-PNP (Ino-HX)
 Native PNP (Guo-Gua)
parametera forward reverse forward Reverse
k(chem), s−1 209 ± 2 152 ± 3 154 ± 3 80 ± 2
ΔH‡, kJmol−1 28 ± 2 86 ± 6 78 ± 3 18 ± 1
TΔS‡, kJmol−1 −32 ± 2 26 ± 2 17 ± 1 −44 ± 2
ΔG‡, kJmol−1 60 ± 5 60 ± 6 60 ± 4 62 ± 4
Ea‡, kJmol−1 30 ± 2 88 ± 6 80 ± 3 20 ± 0.5
a

Determined by fitting the stopped-flow fluorescence rates to the Arrhenius (eq 3) and the Eyring (eq 4) equations.