. Author manuscript; available in PMC: 2010 Apr 28.

Published in final edited form as: Biochemistry. 2009 Apr 28;48(16):3658–3668. doi: 10.1021/bi802339c

Table 5.

Comparison of T-Jump Rate Constants (ms⁻¹) for the Two Equilibrated Systems of F159W-Leuko-PNP with (a) Inosine-PO₄-Hypoxanthine-Ribose1-Phosphate and (b) Hypoxanthine-PO₄

		τ₂⁻¹, ms⁻¹		τ₃⁻¹, ms⁻¹
temp jump	Equilibrated system	k₂^a	k₋₂^a	k₃^b	k₋₃^b
10 to 18 °C	a	6.2 ± 0.6	1.4 ± 0.6	0.50 ± 0.17	0.4 ± 0.2
10 to 18 °C	b	9.0 ±1.6	1.2 ± 0.1	1.20 ± 0.04	0.2 ± 0.1
20 to 27 °C	a	10.5 ± 0.6	1.5 ± 0.6	2.2 ± 0.5	0.9 ± 0.5
20 to 27 °C	b	11.1 ± 2.2	1.3 ± 0.6	2.7 ± 0.1	0.2 ± 0.1
25 to 32 °C	a	10.3 ± 0.1	1.7 ± 0.1	3.1 ± 0.6	1.1 ± 0.7
25 to 32 °C	b	11.8 ± 2.0	2.8 ± 0.3	2.4 ± 0.4	0.6 ± 0.3

^{a, b} Determined by fitting the relaxation rates (τ₂⁻¹ and τ₃⁻¹) to eqs 5 and 6, respectively.