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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Biochemistry. 2009 Apr 28;48(16):3658–3668. doi: 10.1021/bi802339c

Table 6.

Summary of all the Microscopic Rate Constants (Scheme 2) for the Two Equilibrated Systems of F159W-Leuko-PNP at 25 °C

microscopic rates F159W-Leuko-PNP-Ino-PO4-Hx-R1PO4 F159W-Leuko-PNP-Hx-PO4
k1, M−1s−1 (1.2 ± 0.1) × 107
k−1, s−1 178 ± 8
k2, s−1 10,500 ± 600 11,100 ± 2,200
k−2, s−1 1,500 ± 600 1,300 ± 600
k3, s−1 2,200 ± 500 2,700 ± 100
k−3, s−1 900 ± 500 200 ± 100
k4, s−1 209 ± 2 naa
k−4, s−1 152 ± 3 naa
a

Not available.