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. Author manuscript; available in PMC: 2009 Apr 28.
Published in final edited form as: Hum Mol Genet. 2007 Jun 22;16(17):2072–2088. doi: 10.1093/hmg/ddm155

Figure 2.

Figure 2

COMP mice are normal at birth. (A) Skeletal preparations of new born mice (cartilage stained with Alcian Blue and bone with Alizarin Red). There is no overt phenotype in new born mice homozygous or heterozygous for p.Thr583Met and the skeletal elements appeared normal and in proportion for all three genotypes. (B) Von Kossa staining of mineralized bone showed no differences in bone mineralization and morphology between the new born wild-type and p.Thr583Met COMP mice. (C) H&E staining of the growth plate showed no differences in the morphology between the littermates of all three genotypes. (D) IHC using a COMP antibody revealed no differences in the distribution of COMP in the new born WT and mutant growth plates. Scale bar 100 μm. Key: +/+ (WT); m/+ (mice heterozygous for the mutation); m/m (mice homozygous for the mutation); PZ (proliferative zone); PHZ (prehypertrophic zone); HZ (hypertrophic zone).