Table 2.

Accessory factors implicated in MSL targeting

Factor	Protein type	Relevant mutant/RNAi phenotype	References
SET2	H3K36 methyltransferase	Mutation/RNAi reduces MSL binding at active genes on X chromosome, not CES (ChIP of third instar larvae, SL2 cells)	(Larschan et al., 2007; Bell et al., 2008)
GAF (TRL)	GAGA-binding factor	Mutation is associated with an increased number of MSL sites on autosomes and the loss of a single CES (immunostaining of polytenes)	(Greenberg et al., 2004)
UNR	RNA-binding protein	Mutation/RNAi decreases MSL staining on X chromosome (immunostaining of polytenes, SL2 cells)	(Patalano et al., 2009)
NUP153	Nuclear pore protein
MTOR	Nuclear pore-associated protein	RNAi depletion disrupts MSL localization to the X chromosome (immunostaining of SL2 cells)	(Mendjan et al., 2006)

CES, chromatin entry site; ChIP, chromatin immunoprecipitation; RNAi, RNA interference.