Fig. 5.

Effect of mig-6s mutations on MIG-17::GFP distribution. (A) Double heterozygotes of mig-6 and mig-17 display enhancement of class-s DTC defects. Black and gray bars represent percentages of anterior and posterior DTCs, respectively, with defective migrations (total of phase 2D, 2V and 2M defects - see Fig. 2). Lines on the end of the bars indicate standard errors of the expected mean. Numbers of animals scored are indicated towards the right. Asterisks indicate comparisons in which P<0.001 by the chi-square test. (B-E) Localization of MIG-17::GFP is visualized by anti-GFP antibody staining of whole-mount animals (B,D) and frozen cross-sections (green in C,E). Lower panels are Nomarski images of the same animal in the upper panel. MIG-17::GFP in a control genetic background displays uniform distribution on the gonad surface in lateral view (B) and on the gonad, intestine and body wall muscle periphery in cross-section (arrowheads in C). MIG-17::GFP in class-s mig-6(ev700) displays patchy staining along the phase 2 DTC path of a gonad arm (D) and outside of gonad membranes (arrows) in cross-section (E). Staining on the remaining gonad and intestine surfaces is weaker in the mutant genetic background (arrowhead in E) than in the wild type (C) (equal exposure times were used). Eight out of 15 animals in the mutant background showed accumulation of MIG-17::GFP away from the gonad and gut surfaces (arrow), whereas only one of 28 animals in the wild-type background showed this pattern. Phalloidin staining of body wall muscle actin filaments is orange in cross-sections. Positions of intestine (i) and gonad arms (g) are indicated. Scale bars: 20 μm.