Fig. 6.

Effect of collagen IV mutations. (A,B) Temperature-sensitive collagen IV mutant emb-9(g23) cultured at non-permissive temperature from L2 to L4 larval stages showed slow gonad elongation (B), which is similar to class-l defect of mig-6(e1931) (A). Scale bars: 20 μm. (C) Reduction of collagen IV alpha1 by emb-9-specific RNAi (by feeding) or by combining with a heterozygote for the emb-9 null allele suppressed the class-s DTC phenotype of ev701 heterozygotes, as did a mutation of let-2/collagen IV alpha2. Balancer eT1 (aka unc-36) and eT1^* (equals eT1 with a let-500 mutation in the balancing translocation) significantly enhances the penetrance of DTC migration defect in class-s heterozygote for unknown reasons. Black and gray bars represent percentages of anterior and posterior DTCs, respectively, that show defective migrations (total of phase 2D, 2V and 2M defects). Numbers of animals scored are indicated towards the right. Asterisks indicate comparisons in which P<0.001 by the Chi-square test.