Fig. 1.

The Drosophila dap 3′UTR is repressed by miRNAs in the germline stem cells. (A) The control sensor lacks significant 3′UTR. The dapL and dapS sensors contain 866 bp and 630 bp of dap 3′UTR, respectively. Predicted miRNA response elements (MREs) are shown. (B) GFP expression was unchanged in Dcr-1 germline stem cells (GSCs) (arrowhead) in the control sensor background as compared with the neighboring control GSC, but was upregulated in the dapL and dapS sensor lines. (C) The dapL and dapS sensors were upregulated in Dcr-1 GSCs. Mean±s.e.; n≥23 pairs of GSCs. Student's t-test; ^**P<0.01.