The dap 3′UTR responds to InR but not
TGF-β signaling in GSCs. (A) In InR mutant
GSCs (first four rows, arrowhead), the GFP expression is upregulated in
dapL, dapF and dapS, but not in the control sensor. In
punt mutant GSCs (bottom row, arrowhead), the GFP expression in
dapL is not upregulated. (B) Quantification of GFP intensity
in InR, punt or Mad mutant GSCs compared with the
neighboring heterozygous GSCs. The GFP intensity is upregulated in
InR mutant GSCs with dapL and dapF by 1.39-fold and
1.23-fold, respectively. Mean±s.e. for all pairs of GSCs (at least 12
pairs for InRex52.1 clones, five pairs for
punt135 clones, and 24 pairs for Mad12
clones). Student's t-test; **P<0.01.