Fig. 7.

miRNAs and dap act downstream of nutritional/InR signaling to regulate cell division. The average number of progeny in germarial region 1-2A counted under conditions of differing nutrition. (A) The cell division of FRT82B control GSCs was reduced dramatically under poor food as compared with rich food conditions, whereas the cell division of Dcr-1 GSCs was not significantly reduced. (B) The cell division of control GSCs was dramatically reduced under poor food as compared with rich food conditions, whereas cell division of dap GSCs was not significantly reduced. The cell division of dap⁴ GSCs was not significantly increased compared with FRT42B control GSCs in poor food conditions (P=0.14075, Student's t-test). (C) Reduction of dap⁴ partially rescues cell cycle defects in InR-deficient GSCs. Other dap alleles gave similar results (CyO/+;InR^ex52.1, 50% division index; dap^2x10/+;InR^ex52.1, 68.75% division index). Mean±s.e. of three repeats. Student's t-test; ^*P<0.05, ^**P<0.01. n=∼40 per condition.