Fig. 6.

Mutant Vangl2^Lp grafted postnatal uterine tissue displays hyperpolarized actin and abnormal E-cadherin distribution. (A-F) The wild type (A-C) has a thin and even domain of actin polarization towards the lumen, as compared with the Vangl2^Lp mutant (D-F) in which the actin staining (red) is uneven (compare B with E, arrows). E-cadherin (green) is enriched at the apical domain of lateral cell junctions in wild-type epithelium (C, arrows), but this localization is lost in the mutant (F, arrows). (G-L) Scrb1 localization is perturbed in grafted postnatal Vangl2^Lp mutant uterine tissue. The wild type (G,J) demonstrates discrete points of Scrb1 localization (green) to apical regions (lumen to the left in G,J) of epithelial cell-cell contact (J, arrows). By contrast, in Vangl2^Lp heterozygotes (H,K,I,L), the expanded pseudostratified layer of epithelial cells localize Scrb1 in a non-polarized fashion, including some Scrb1⁺ epithelial cells in the middle of the lumen (H, arrowhead) and uniform membrane localization (H,I,K,L). Scrb1 localization is abnormal in the highly pseudostratified Vangl2^Lp/Lp mutant epithelium (I,L). (M-O) Increase in smooth muscle layer with corresponding decrease in number of mesenchymal cells in Vangl2^Lp mutants. Wild type (M), Vangl2^Lp/+ (N) and Vangl2^Lp/Lp (O) grafted mouse uterine postnatal tissue sections were stained for laminin (green) and smooth muscle actin (red). Laminin delineates the basal lamina at the border between epithelium and mesenchyme. Double-headed arrows indicate the width of the mesenchyme (M,N). The homozygous mutant (O) displays smooth muscle directly adjacent to the basal lamina (no mesenchyme). Scale bars: 10 μm in G-I,M-O; 5 μm in A-F,J-L.