Table 1.
Phenotypic characterization of postnatal grafted tissue
| Genotype | Glands | Pseudostratified | Vesicles | Mesenchyme to muscle | Cell protrusions |
|---|---|---|---|---|---|
| WT | 4 | 1 | 1 | >> | − |
| WT | 14 | 2 | 2 | − | |
| WT | 1 | 1 | 1 | = | − |
| Average | 6.3 | 1.3 | 1.3 | n.d. | |
| Lp/+ | 0 | 2 | 3 | = | ++ |
| Lp/+ | 10 | 2 | 0 | n.a. | − |
| Lp/+ | 3 | 1 | 1 | < | − |
| Lp/+ | 3 | 2 | 2 | < | − |
| Lp/+ | 1 | 2 | 2 | <<< | − |
| Lp/+ | 0 | 2 | 0 | <<< | − |
| Lp/+ | 0 | 3 | 3 | = | − |
| Lp/+ | 0 | 2 | 2 | n.a. | − |
| Lp/+ | 0 | 0 | 0 | <<< | − |
| Lp/+ | 0 | 1 | 1 | << | − |
| Lp/+ | 0 | 2 | 1 | = | − |
| Lp/+ | 4 | 1 | 1 | n.a. | − |
| Average | 1.8 | 1.6 | 1.3 | n.d. | |
| Lp/Lp | 3 | 4 | 3 | <<< | − |
| Lp/Lp | 2 | 3 | 1 | < | − |
| Lp/Lp | 10 | 3 | 3 | < | ++ |
| Lp/Lp | 8 | 3 | 0 | <<< | − |
| Lp/Lp | 2 | 3 | 2 | << | − |
| Lp/Lp | 1 | 2 | 3 | n.a. | − |
| Lp/Lp | 4 | 2 | 2 | < | ++ |
| Average | 4.3 | 2.8 | 2.0 | n.d. |
Vangl2Lp mutant grafts have an increased index of abnormal phenotypes as observed by H&E staining. Column headings refer to histological phenotypes observed by microscopic observation of the entire section of H&E-stained grafted tissue and designate the following: Glands, the total number of glands; Pseudostratified, the degree of pseudostratified epithelium; Vesicles, lipid vesicles or vacuoles of increased size and/or number; Mesenchyme to muscle, the proportion of total mesenchyme versus total muscle (> indicates more mesenchyme than muscle); Cell protrusions, epithelial cell protrusions or extensions projecting into the lumen. The phenotypic index was determined by calculating the average value for each genotype as shown in the `average' row.
Lp, Vangl2Lp mutation; WT, wild type; n.a., not applicable; n.d., not done.