Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Food Chem Toxicol. 2008 Nov 6;47(1):198–204. doi: 10.1016/j.fct.2008.10.029

Figure 5.

Figure 5

Effects of resveratrol treatment on the accumulation of carbonylated proteins and proteasome activity in pTM cells after chronic oxidative stress. Total protein carbonylation and proteolitic activity were measured in cells incubated at 40% oxygen treated with either resveratrol or vehicle and compared to cells incubated at 5% oxygen. Representative Western Blot from oxidized proteins is shown in panel A (1= negative control for carbonylation; 2= DMSO/ 5% oxygen; 3= DMSO/ 40% oxygen; 4= resveratrol/ 40% oxygen) and total proteins stained with Commasie Blue in panel B. Densitometry summarizing 3 different experiments is showed in panel C. Panel D shows the proteolytic activities of the proteasome subunits: CT-L (chymotrypsin like), T-L (trypsin like), and PGHP (caspase like) (N=3, t-test, * P ≤ 0.05; # Kruskal-Wallis test P ≤ 0.049).