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. Author manuscript; available in PMC: 2009 Apr 28.
Published in final edited form as: Methods Enzymol. 2008;450:107–127. doi: 10.1016/S0076-6879(08)03406-X

Figure 2.

Figure 2

(A) Schematic drawing of the adapter used for spectral FRET measurements of TM helix dimerization in surface supported bilayers. The adapter fits in the cuvette holder of a standard fluorometer. A fluid bilayer is supported on the coverslip, and is sandwiched between the coverslip and the quartz slide. The gap between the coverslip and the quartz slide is filled with buffer, such that the bilayer is fully hydrated. (B) Top view showing the bilayer, supported on the coverslip, and the quartz slide (not drawn to scale). Reprinted with permission from (Merzlyakov et al 2006). Copyright (2006) American Chemical Society.