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. Author manuscript; available in PMC: 2009 Apr 28.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Aug 31;226(2):107–118. doi: 10.1016/j.taap.2007.08.018

Figure 2.

Figure 2

Effect of TCDD administration for 8 or 16 weeks on the NADPH-dependent 2- and 4-hydroxylation of E2 by liver microsomes of female C3H/HeN mice. The animals (5 − 6 animals per group) were injected i.p. with TCDD at 1, 10, or 100 μg/kg b.w. (dissolved in 100 μL corn oil) once every two weeks for 8 weeks or at 1 or 10 μg/kg b.w. for 16 weeks; the control animals were injected the same volume of corn oil alone. The collected livers from 5 − 6 animals in each treatment group were randomly pooled into two batches (with 2 − 3 livers/batch), and thus two separate batches of liver microsomes were prepared for each treatment group. The assay conditions and the measurement of the catechol estrogens by HPLC were described in the Materials and Methods section. Each bar represents the mean ± S.D. of triplicate measurements of each pooled microsomal preparation. An asterisk placed on top of a bar denotes that the difference is statistically significant (P < 0.05, Student's t-test) when it was compared to any of the two control values (without TCDD treatment).