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. Author manuscript; available in PMC: 2009 Apr 28.
Published in final edited form as: Virology. 2007 Oct 31;371(2):336–349. doi: 10.1016/j.virol.2007.09.030

FIGURE 1. Noc interfered with BKV infection.

FIGURE 1

FIGURE 1

HRPTEC were pre-incubated with Noc (6.25, 12.5, 25, and 50 μM) for 1 hour prior to co-incubation with BKV (MOI 0.5 FFU/cell) and Noc. After 72 hours medium was removed, cells were washed three times with REBM with 0.5 % FBS and incubated for another 48 hours with fresh medium containing Noc. (A) After incubation, cells were fixed and analyzed by IF (Magnification ×20). T-Ag positive cells were counted as BKV infected cells and DAPI stained nuclei were counted as total cells. Untreated HRPTEC (control) and HRPTEC incubated with only BKV (BKV) were used as negative and positive controls. At least 500 cells were counted from each three independent cover slips and means and SE were calculated from two independent experiments. *: P<0.001. (B) After incubation, cells were harvested and analyzed by WB. Relative T-Ag expression was expressed as graph bars following measurement of band intensity by Odyssey®. Relative intensity of T-Ag expression was normalized using the intensity of GAPDH as loading control. Means and SE were calculated from two independent experiments. *: P<0.001.