Figure 3.

Quantification of absolute levels of egr-1 expression in (a) visual areas, (b) song nuclei, (c) motivation-related areas and (d) motor-associated and auditory areas, contralateral to open (top bars) and covered (bottom bars) eyes. n=5 for female-directed singing (FD, grey bars); n=5 for silent alone (SA, white bars); n=3 for silent dark (SD, black bars). Each bar shows an average value±s.e.m. ^*p<0.05, ^**p<0.01, ^***p<0.001. The asterisks inside the bars are significant differences between hemispheres within a group; the asterisks above or below the bars are significant differences among groups (two-way repeated-measures ANOVA; hemisphere F=1.782–92.382; group F=1.015–22.672). In some comparisons of the ANOVA, the accepted α-level was less than 0.05, and, in these cases, we indicated only the significance with the appropriate asterisk if the p-value was below that α-level cut-off.