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A, LNCaP cells transfected with siRNA against NKX3.1 or scramble siRNA were infected with different doses of RalA Q72L dominant active retrovirus or LacZ control retrovirus for 48 h. Total RNA was collected and subjected to real-time PCR using primers for VEGF-C and 36B4 internal control. The data presented here represents the mean of three individual experiments. B, schematic diagram detailing the molecular mechanisms of androgen-regulated VEGF-C transcription in prostate cancer.
