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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Microbiology (Reading). 2008 Sep;154(Pt 9):2767–2775. doi: 10.1099/mic.0.2008/019729-0

Figure 2.

Figure 2

(A) The HOM3 and THR1 genes were placed under the control of the PCTR4-1 promoter by inserting the NAT1-PCTR4-1 construct immediately upstream of the predicted ORF, as shown in the diagram. (B) Southern hybridization analysis confirming correct positioning of the NAT1-PCTR4-1 construct in strains H99-73 (PCTR4-1-THR1) and H99-76 (PCTR4-1-HOM3). Genomic DNA from strains H99 (wildtype), H99-73 and H99-76 was digested with the restriction enzymes indicated, and blots were hybridized as indicated with a HOM3 or THR1 DNA probe, amplified using primer pairs JO414+JO413 and JO506+JO362, respectively.