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. 2009 Apr 28;106(19):7921–7926. doi: 10.1073/pnas.0902191106

Fig. 4.

Fig. 4.

Effects of HP and hormone treatments on hFIX expression in transgenic mice carrying a hemophilia B Leyden phenotype minigene, -802(-20A) FIXm1. (A) Effects of HP and a course of administration of GH and DHT, followed by the second course of GH administration, on hFIX expression in a male mouse P53-m6(F2/m) carrying -802(-20A)FIXm1. HP was done at 6 months of age. Vertical arrows show time points for HP, initiation and termination of GH, DHT, and the 2nd GH administration. Vertical axis: serum hFIX concentration level; horizontal axis: age and duration of various treatments in month or day. (B) Effects of HP and subsequent administrations of PBS, sesame oil, and GH, on hFIX expression in a male animal P53-m10(F2/m) carrying -802(-20A) FIXm1. The rest are the same as in (A). (C) Northern blot analyses of mouse liver hFIX mRNA preparations. Total liver RNA samples (15 μg per lane) were used for analyses. Lane 1, wild-type animal; lane 2–4, Leyden phenotype animals carrying -802(-20A)FIXm1. Lane 2, animal P127(F1/f) without HP; lane 3, animal P126(F1/f) after HP; lane 4, P108(F1/f) animal sacrificed immediately after the course of GH injection at eighth day. (Upper) Filter probed with the SspI/BamHI fragment of hFIX cDNA; (Lower) filter re-hybridized with RNR18 probe (1). A horizontal arrow on the right indicates the hFIX mRNA band position with size information.