Table 1.

Testing for consistent within-species and within-lineage variations in nucleotide substitution rates (N: non-synonymous, S: synonymous) of the MHC-DRB molecule at different regions (ABS: contact residues, non-ABS: non-contact residues)

All lineages	Species-specific effects	Lineage-specific effects
dN, ABS	F42,148 = 2.455, P < 0.001	F59,131 = 1.994, P < 0.001
dS, ABS	F42,148 = 1.628, P = 0.018	F59,131 = 1.544, P = 0.021
dN, non-ABS	F42,148 = 1.450, P = 0.055	F59,131 = 1.490, P = 0.031
dS, non-ABS	F42,148 = 1.893, P = 0.003	F59,131 = 2.568, P < 0.001
dN, all sites	F42,148 = 2.227, P < 0.001	F59,131 = 1.744, P = 0.005
dS, all sites	F42,148 = 1.503, P = 0.040	F59,131 = 1.782, P = 0.003
Only HLA othologous	Species-specific effects	Lineage-specific effects
dN, ABS	F20,34 = 2.260, P = 0.018	F16,38 = 0.798, P = 0.679
dS, ABS	F20,34 = 1.632, P = 0.102	F16,38 = 0.360, P = 0.984
dN, non-ABS	F20,34 = 2.195, P = 0.021	F16,38 = 1.412, P = 0.188
dS, non-ABS	F20,34 = 2.187, P = 0.022	F16,38 = 0.380, P = 0.980
dN, all sites	F20,34 = 2.765, P = 0.004	F16,38 = 0.783, P = 0.694
dS, all sites	F20,34 = 2.329, P = 0.015	F16,38 = 0.310, P = 0.993

Results form one-way ANOVA, when including all lineages and only human orthologues (i.e. after excluding W lineages).