Figure 1.

PTEN induces a G₁ block. (A) PTEN, but not pVHL or cdc25C, induced a G₁ block in 786-O cells. 786-O cells were transiently cotransfected with a plasmid encoding CD19 (pCD19) along with plasmids encoding the indicated proteins. Forty hours after transfection, cells were fixed and the cell-cycle distribution of the successfully transfected cells was determined by fluorescence-activated cell sorting analysis. The mean and SEM of two experiments are shown. (B) PTEN does not alter the cell-cycle profile of SAOS-2(RB−/−) cells. SAOS-2 cells were transiently transfected with pCD19 and the plasmids encoding the indicated proteins and analyzed as in Fig. 2A. The mean and SEM of two experiments are shown. (C) PTEN does not alter the cell-cycle profile of ACHN cells. ACHN cells were transiently transfected with pCD19 or plasmids encoding the indicated proteins and analyzed as in A. The mean and SEM of two experiments are shown. (D) Immunoblot detection of PTEN protein in 786-O, SAOS-2, and ACHN cells. C54 anti-PTEN antiserum was used to detect PTEN by immunoblot analysis of protein extracts from the indicated cell lines.