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Cells lacking PTEN protein contain high levels of Akt, phosphorylated on Ser-473. The indicated cells lines were serum-deprived for 24 h in duplicate. One p100 plate from each pair was refed with fresh medium containing 10% fetal calf serum for 90 min, after which protein extracts were prepared and subject to immunoblot analysis with an antibody directed at Akt phospho-Ser-473 (Upper) or an antibody that recognizes Akt independent of its phosphorylation status (Lower).
