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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Gene. 2009 Feb 10;436(1-2):108–114. doi: 10.1016/j.gene.2009.01.020

Figure 4. Hbo1 regulates global H4 acetylation.

Figure 4

(A) Whole cell extracts infected with Ad-GFP (“GFP”) and Ad-antisense Hbo1 (“AS”) for 48 hr were analyzed by western blotting with antibodies to Hbo1, acetylated histone H4, and α-tubulin. (B) Whole cell extracts infected with Ad-Hbo1 (“WT”) and Ad-catalytically inactivated Hbo1 (“Mut”) for 48 hr were analyzed by western blotting with antibodies to Hbo1, acetylated histone H4, and α-tubulin. (C) Chromatin fractions of virus-infected MCF7 cells were separated on a protein gel, transferred to a membrane, were assayed for Histone H2B by immunoblotting to examine integrity of core histones.