Fig. 3.

The effect of WP1066 on the cytotoxicity of CD8+ T cells against human melanoma A375 cells. A375 cells labeled with CFSE were exposed to multiple experimental conditions in the presence or absence of CD8+ T cells from healthy donors (effector:target cell ratio = 5:1). Cells were grown in triplicate cultures in RPMI 1640 medium alone or supplemented with 2 μM of WP1066, CD8+ T cells, or CD8+ T cells plus 2 μM of WP1066. Flow cytometry was used to determine the percentage of PI-positive cells for each experimental condition. There were no statistically significant differences between the CD8+ T cell group and CD8+ T cell plus WP1066 group. These experiments were reproduced four times with similar results