Figure 1.

Effects of hyaluron (HA) species on mRNA expression of ADAMTS species in interleukin (IL)1α-stimulated osteoarthritic chondrocytes. (A) Reverse transcription-PCR analysis of the expression of ADAMTS species and tissue inhibitor metalloproteinase 3 (TIMP3). Total RNA was isolated from the chondrocytes which were treated for 24 h without (Cont) or with IL1α (1 ng/ml) and HA (250 μg/ml for HAoligo and 2.5 mg/ml for other HA species), and reverse-transcribed into cDNA followed by PCR. The experiments were carried out in triplicate, and representative data are shown. Positive controls (PC) are rheumatoid synovial fibroblasts for ADAMTS1, ADAMTS5 and TIMP3, U-251 human glioblastoma cells for ADAMTS4, ADAMTS9 and ADAMTS15, and CaR-1 rectal carcinoma cells for ADAMTS8.¹⁰ (B) Real-time PCR analysis of the mRNA expression of ADAMTS4. Relative expression levels of ADAMTS4 in osteoarthritic chondrocytes treated without (Cont) or with IL1α and HA species were normalised to an endogenous control, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars are mean (SD) (n = 5). *p<0.05; **p<0.01; ***p<0.001.