Figure 2.

Characteristic [cAMP] changes in preBötC neurons. Shown are representative responses (for the statistics, see Table 1) due to modulation of activity of adenylate cyclase, phosphodiesterase and activation of metabotropic receptors. AC was stimulated with forskolin and inhibited with 2'5'-dideoxyadenosine (DDA), PDE was antagonized with IBMX and rolipram, and Group I metabotropic glutamate receptors were activated with (S)-3,5-dihydroxyphenylglycine (DHPG). Fluorescence signals were averaged over the soma of neurons and transformed into cAMP concentrations as described in Methods.