Figure 1.

Restoration of peroxisomes in CG-J CHO mutant cells. (a) Peroxisome-deficient mutant ZP119 cells. (b) Peroxisome-restored ZP119, after lipofection with a combined pool (F6-19) of human cDNA library. Arrows indicate the complemented cells. Cytosolic appearance of catalase was apparent in the other cells. (c and d) 119P19 cells, stable HsPEX19-transformants of ZP119 cells. Cells were stained with antisera to catalase (a and b), PTS1 peptide (c), and PMP70 (d), respectively. (Magnification: ×630; bar = 20 μm.)