Table 2.
Complementation of CHO cell mutants and patient fibroblasts by HsPEX19
| CHO mutant | Peroxisomepositive clone | Patient fibroblasts from CG | Peroxisomepositive | Gene |
|---|---|---|---|---|
| ZP119 | 24/30 | J (PBDJ-01) | + | PEX19 |
| ZP165 | 21/30 | |||
| ZP107 | − | E (I) | − | PEX1 |
| ZP139 | − | II | − | PEX5 |
| ZP109 | − | III | − | PEX12 |
| ZP92 | − | C (IV) | − | PEX6 |
| (VI) | ND | |||
| B (VII) | − | PEX10 | ||
| A (VIII) | − | |||
| D (IX) | − | PEX16 | ||
| Z65 | − | F (X) | − | PEX2 |
| G | − | |||
| ZP110 | − | |||
| ZP114 | − |
Peroxisome-deficient CHO mutants of 8 CGs (4–8, 13–18) and fibroblasts from 10 CG PBD patients (3, 4, 8, 9) were transfected with pUcD2Hyg⋅HsPEX19 and examined for peroxisome assembly by immunostaining with antisera to rat and human catalase, respectively, at 3 days after transfection. Parentheses indicate fibroblasts of CG not used in this experiment. In CG-J ZP119 and ZP165, peroxisome-positive colonies were counted in 30 colonies; in other cells: +, complemented; −, not complemented; ND, not determined.