Figure 2.

Hepatic glucocorticoid metabolism. 11β-HSD1 activity measured as velocity of formation of product following incubation of [³H]₄-corticosterone with hepatic homogenate from (A) Db/? control (open) or db/db mice (light striped); (B) Ob/? control (black) or ob/ob mice (dark striped). 5β-Reductase activity measured as velocity of formation of product following incubation of [³H]₄-corticosterone with hepatic cytosol from (C) Db/? control or db/db mice; (D) Ob/? control or ob/ob mice. Abundance of mRNAs for hepatic enzymes measured by real-time PCR (corrected for 18S as a housekeeping gene and presented as a percentage of respective control group) in (E) Db/? control or db/db mice; (F) Ob/? control mice or ob/ob mice. Data are mean±s.e.m.; n=10–12/group; *P<0·05; **P<0·01.